Beyond ribose and phosphate: Selected nucleic acid modifications for structure–function investigations and therapeutic applications

• Christopher Liczner,
• Kieran Duke,
• Gabrielle Juneau,
• Martin Egli and
• Christopher J. Wilds

Beilstein J. Org. Chem. 2021, 17, 908–931, doi:10.3762/bjoc.17.76

• targeted to proviral HIV DNA [68], and as a triplex-forming oligonucleotide that selectively binds a sequence within the chromatin structure of cell nuclei [69]. Remarkably, 3'-NP DNA can also act as an RNA mimic in interactions with binding proteins despite lacking a ribose moiety, making them useful

Design and biological characterization of novel cell-penetrating peptides preferentially targeting cell nuclei and subnuclear regions

• Anja Gronewold,
• Mareike Horn and
• Ines Neundorf

Beilstein J. Org. Chem. 2018, 14, 1378–1388, doi:10.3762/bjoc.14.116

• described as beneficial tools in the creation of anticancer drugs [21]. Within this study, we aimed to design novel efficient cell-penetrating peptides that preferentially locate within cell nuclei and subnuclear regions. For this, we generated peptide chimera consisting of a shortened version of the

• also accumulated in the cell nuclei and nucleoli. This observation supported the idea of cellular entry via direct penetration when using a concentration of 10 µM. In MCF-7 cells, NrTP-sC18* was also evenly distributed throughout the whole cell, including strong accumulation in the nuclei. Notably, N50

• time. Cells were incubated at 4 °C or 37 °C and 10 min prior to the end of incubation 0.6 μL of Hoechst stain (bisbenzimide H33342, 1 mg/mL in H2O, sterile filtered) was added to each well to stain the cell nuclei. After removing the solutions, cells were quenched with 200 μL of 150 μM trypan blue

A journey in bioinspired supramolecular chemistry: from molecular tweezers to small molecules that target myotonic dystrophy

• Steven C. Zimmerman

Beilstein J. Org. Chem. 2016, 12, 125–138, doi:10.3762/bjoc.12.14

• sequestration. However, 27 did not appear to enter cells easily and was poorly soluble and cytotoxic. Amin Jahromi recognized that acridine-containing compounds had been previously induced to enter cells and cell nuclei by attaching amino groups that take advantage of a polyamine transporting system [84][85

Come-back of phenanthridine and phenanthridinium derivatives in the 21st century

• Lidija-Marija Tumir,
• Marijana Radić Stojković and
• Ivo Piantanida

Beilstein J. Org. Chem. 2014, 10, 2930–2954, doi:10.3762/bjoc.10.312

• cellular uptake (Figure 11), upon which red colour of 19 accumulated in cell nuclei – intriguingly after only 2 hours fluorescence colour changed to yellow (Figure 11, right) and the dye distributed over the cytoplasm pointing out to the metabolic modification of the compound. The new, easily accessible

SF002-96-1, a new drimane sesquiterpene lactone from an Aspergillus species, inhibits survivin expression

• Silke Felix,
• Louis P. Sandjo,
• Till Opatz and
• Gerhard Erkel

Beilstein J. Org. Chem. 2013, 9, 2866–2876, doi:10.3762/bjoc.9.323

• glass-slide, covered with a coverslip and the morphology of the cell nuclei was observed by using a fluorescence microscope (Zeiss, Axioskop). Structure of SF002-96-1. COSY (bold) and HMBC (arrow) correlations of SF002-96-1. NOESY correlations of SF002-96-1. Effect of SF002-96-1 on survivin promoter

• ) Detection of DNA fragmentation by agarose gel electrophoresis after treatment of Colo 320 cells for 5 h with different concentrations of the test compound. Control: untreated cells only. (C) Morphology analysis of the cell nuclei by DAPI staining after treatment of Colo 320 cells with 26.31 µM of SF002-96-1